谷氨酸诊断/测定试剂盒及谷氨酸浓度测定方法

Glutamic acid diagnosis/determination reagent kit and method for determining glutamic acid concentration

Abstract

本发明涉及一种利用酶比色法及酶联法技术的谷氨酸诊断/测定试剂盒,同时本发明还涉及测定谷氨酸浓度的方法原理、试剂的组成及成分,属于医学/食品检验测定技术领域。本发明的试剂盒主要成分包括:缓冲液、还原型辅酶、谷氨酸氧化酶、NADH过氧化物酶及稳定剂;通过将样品与试剂按一定的体积比混合,使之发生一系列的酶促反应,再将反应物置于紫外/可见光分析仪下,检测主波长340nm处吸光度下降的程度/速度,从而测算出谷氨酸的浓度大小。
The invention relates to a kit for diagnosing/mensurating glutamic acid by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the glutamic acid, and belongs to the technology field of medical/food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamate oxidase, NADH peroxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the glutamic acid.

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